Expression of collagen type I and type II after articular cartilage injury

doi:10.3969/j.issn.2095-4344.2014.51.020

Abstract

Abstract:

BACKGROUND: The relationship between collagen type I and osteoarthritis is still controversial, and expression patterns of collagen type I are not yet fully understood. There is lack of relevant research addressing the above-mentioned issues.
OBJECTIVE: To investigate the patterns of expression of collagen type I, and to contribute to the roles of collagen type I in the pathogenesis of osteoarthritis.
METHODS: The articular cartilage injury model was created in 10 New Zealand white rabbits. A 5 mm longitudinal wound was made in the middle of femoral trochlear groove using a scalpel. At 2 and 6 weeks after modeling, five animals were sacrificed, respectively. The injured articular cartilage was taken to make paraffin sections. Immunohistochemistry detection was used to detect the expression of collagen type I and type II around the injury. Safranine O-fast green staining was used to detect the distribution of proteoglycan around the injury.
RESULTS AND CONCLUSION: Results from the immunohistochemistry detection showed that a small amount of collagen type I was detected around the injury at 2 weeks after modeling, and there was much more collagen type I around cartilage injury at 6 weeks after modeling, but collagen type II had no obvious changes. Under the safranine O-fast green staining, no remarkable degeneration was found around the injured cartilage. These findings indicate that collagen type I has been increased in the early period of osteoarthritis. Expression of collagen type I is increased during the degeneration of articular cartilage, indicating a close relationship between collagen type I and osteoarthritis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: collagen type I, collagen type II, knee joint, cartilage, rabbits

CLC Number:

R318

Zhang Meng, Zhou Jia-jun, Luo Zong-ping. Expression of collagen type I and type II after articular cartilage injury[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(51): 8305-8309.

Figures/Tables 3

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